84-3423-21 ［取扱停止］SFRP4 (Secreted Frizzled Related Protein 4, DDC-4, FrpAP, frpHE, FrzB-2) 100ug S1012-89-AF
特徴
- sFRP4 belongs to the sFRP family whose members contain a cysteine-rich domain homologous to the Wnt-binding domain of the Frizzled proteins.
- SFRP4 is a circulating protein that can function as a phosphatonin as well as an antagonist of Wnt signaling.
- Applications:Suitable for use in Direct ELISA and Western Blot.
- Other applications not tested.
- Recommended Dilution:Direct ELISA: 0.5-1.0ug/ml.
- Detection limit for rhsFRP-4 is ~2ng/well
- Western Blot: 0.1-0.2ug/ml.
- Detection limit for rhsFRP-4 is ~20ng/lane under non-reducing and reducing conditions
- Optimal dilutions to be determined by the researcher.
- Storage and Stability:May be stored at 4°C for short-term only.
- For long-term storage, store at -20°C.
- Aliquots are stable for at least 24 months at -20°C.
- For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Further dilutions can be made in assay buffer.
仕様
- Size：100ug
- Host：goat
- Source Antibody：human
- Grade：Affinity Purified
- Purity：Purified by immunoaffinity chromatography. Endotoxin: < 0.1 EU per 1ug antibody (LAL method).
- Form：Supplied as a liquid in PBS, pH 7.2 Also available with trehalose see S1012-89.
- Specificity：Recognizes human sFRP-4. Shows < 5% crossreactivity with rhsFRP-3, rhsFRP-1 and rmsFRP-2.
- Isotype：IgG
- Calc Applications Abbrev：E WB
- Calc Crossreactivity：Hu
- Immunogen：NS0-derived rhsFRP-4
- Shelf Life：1year
- Swiss Prot Number：6424
- EU Commodity Code：30021010
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