84-3423-17　VEGF (Vascular Endothelial Growth Factor) 30ug　301786

特徴

• Vascular endothelial growth factor (VEGF) is a dimeric glycoprotein that stimulates vasculogenesis and angiogenesis.
• VEGF belongs to a family of closely related growth factors that share a conserved pattern of eight cysteine residues and bind to common VEGF receptors.
• The VEGF family members bind in an overlapping manner to three receptor tyrosine kinases, VEGFR1, VEGFR2 and VEGFR3.
• Specifically, VEGF mediates angiogenic response by the activation of VEGFR2.
• VEGF is produced at high levels in endothelial cells but its expression can be found in other cell types, including fibroblasts, inflammatory cells, and many tumor cells, often in response to increasing tumor hypoxia via the HIF-1a pathway.
• Overexpression of VEGF can contribute to diseases such as cancer and vascular disease in the retina; also, VEGF is a cause of susceptibility to microvascular complications of diabetes type 1 such as diabetic retinopathy, diabetic nephropathy and diabetic neuropathy.
• Applications:Suitable for use in Immunofluorescence, Western Blot.
• Other applications not tested.
• Recommended Dilution:Immunofluorescence: 1:500-1:2000 Western Blot: 1:1000-1:8000Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.