84-3423-12　Tau Cleaved by Caspase-6 50ul　301772

特徴

• The Tau protein is a microtubule-associated protein mainly expressed in neurons and plays a crucial role in the neuronal cytoskeleton stabilization.
• They are abundant in neurons of the central nervous system and are less common elsewhere, but are also expressed at very low levels in CNS astrocytes and oligodendrocytes.
• Defects in tau proteins can result in dementias, such as Alzheimer's disease (AD).
• AD is a neurodegenerative pathology which is characterized by the presence of two types of neuropathological hallmarks: neurofibrillary tangles (NFTs) and senile plaques (SPs).
• NFTs are intraneuronal aggregations mainly composed of abnormally phosphorylated Tau.
• In neuropil threads (NPTs), NFTs, and neuritic plaques (NPs) of severe Alzheimer’s disease (AD) brains highly abundant amounts of active Caspase 6 and Tau cleaved by Caspase 6 (Tau delta Csp6) have been found.
• Caspases are a family of cytosolic aspartate- specific cysteine proteases.
• Sequential activation of caspases plays a central role in the execution phase of cell apoptosis.
• Caspase 6 has been associated to increase levels of b-amyloid peptide in primary cultures of human neurons.
• Caspase 6 has also been demonstrated to be active in NPTs, NFTs, and NPs in the hippocampus and temporal cortex in sporadic AD.
• Caspase 6 activation has been suggested to be an early event in AD and to precede the development of frank lesions.
• The main role of tau proteins is to stabilize microtubules.
• Tau proteins can be mainly found in neurons, in the central nervous system.
• Applications:Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and immunocytochemsitry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:100-1:500Immunohistochemistry: 1:2000-1:10,000Immunoprecipitation: 1:100-1:500 Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.