The Interferon-inducible double stranded RNA-dependent protein kinase activator, also known as PKR-associated protein X (RAX in mouse) or PACT (in human) is a cellular protein that heterodimerizes with PKR through its double-stranded RNA binding proteins (dsRBDs).
PKR is an interferon (IFN)-induced serine-threonine protein kinase that plays a central role in IFN's antiviral and antiproliferative activities.
In the absence of viral infections, PACT activates PKR in response to diverse stress signals as serum starvation and treatments with arsenite or peroxide, resulting in the induction of the apoptotic cascade.
PACT is expressed in most cell types at a very low abundance, and overexpression of PACT causes PKR activation, leading to eIF2α phosphorylation.
PACT has been shown to interact with another DRBM-containing protein, TRBP (TAR RNA binding protein), and form a triple complex with Dicer to facilitate the production of siRNA.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:500Western Blot: 1:1000-1:3000Immunoprecipitation: 10ugOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a powder lyophilized from a solution containing PBS, pH 7.4. No preservative added. Reconstitute with 50ul ddH2O.
Specificity：Recognizes human PACT and the mouse homologue RAX.
