Nucleobindin 1 is a 461aa ubiquitous protein with an N-terminal of 25aa signal peptide responsible for its initial localization to the endoplasmic reticulum (ER).
It has also been detected in the cytoplasm and in various subcellular structures such as the nucleus, mitochondria and the Golgi apparatus.
Nucleobindin plays a role in a number of cellullar processes such as intracellular signaling, inflammation, autoimmunity, osteogenesis and cancer.
Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:150Western Blot: 1:1000Immunocytochemistry: 1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder. No preservative added. Reconstitute with 100ul sterile ddH2O.
Specificity：Recognizes human Nucleobindin. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IF IHC IP WB
Calc Crossreactivity：Hu Mo
Immunogen：Full-length recombinant protein corresponding to human Nucleobindin.
