The LDL Receptor (LDLR) is a transmembrane protein of 839aa that mediates the endocytosis of cholesterol-rich LDL.
The LDL receptor plays a critical role in cholesterol homeostasis.
It binds, internalizes, and removes LDL from serum.
LDLR recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles, as well as the apoE protein found in chylomicron remnants and VLDL remnants (IDL).
To be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits (or buds) on the cell surface.
Defects in LDLR are the cause of familial hypercholesterolemia, a common autosomal semi-dominant disease that affects about 1 in 500 individuals.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1500Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Ascites
Purity：Ascites
Form：Supplied as a lyophilized powder. No preservative added. Reconstitute with 100ul ddH2O.
Specificity：Recognizes the C-terminal portion of rat LDLR.
