SamD10 and SamD12 are two recently discovered homolog proteins consisting of a SAM domain.
These proteins are also know as Hyphen (HYP) or Aveugle (Ave) and they have been demonstrated to interact with the SAM domain of the Connector enhancer of KSR (CNK) downstream of RAS but a a step upstream of RAF.
A role for these proteins downstream of EGFR during eye and wing development of Drosophila has also been demonstrated.
Heterodimerization of CNK/SAM has been shown to facilitate the recruitment of a KSR/MEK complex to CNK and bridge KSR/MEK to RAF which greatly enhances RAS-mediated RAF activation.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Ascites
Purity：Ascites
Form：Supplied as a lyophilized powder. Reconstitute with 100ul ddH2O.
Specificity：Recognizes SamD10 and SamD12. Species Crossreactivity: Human, mouse
