HMG-CoA reductase (also known as 3-hydroxy-3-methyl-glutaryl-CoA reductase or HMGCR) is a transmembrane glycoprotein involved in the control of cholesterol biosynthesis.
It is the rate- limiting enzyme of sterol biosynthesis; it is a ubiquitously expressed glycoprotein that is bound to the membrane of the endoplasmic reticulum.
HMG-CoA reductase is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase.
HMG-CoA is inhibited by statin, a class of cholesterol-lowering drugs which lower plasma cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor.
Applications:Suitable for use in Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:50-1:100Western Blot: 1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Ascites
Purity：Ascites
Form：Supplied as a lyophilized powder. Reconstitute with 100ul ddH2O.
Specificity：Recognizes rat HMG CoA reductase.
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Rt
Immunogen：Full length protein corresponding to rat HMG CoA reductase.
