Dicer is an endoribonuclease which has two RNase III domains and one PAZ domain and belongs to the highly conserved family of dsRNA-specific endoribonucleases RNase III family.
Dicer plays important roles in RNA processing, post-transcriptional gene expression control and defense against viral infection.
Synthesis of proteins can be blocked by RNA in a process known as RNA interference and for this process to happen; three important events have to occur.
First, DICER processes dsRNA into smaller fragments known as short interfering RNAs (siRNAs) and microRNAs (miRNAs).
Second, a dsRNA binding protein recruits a DICER-siRNA complex to Argonaute2 (Ago2), which is an endonuclease capable of degrading messenger RNA (mRNA).
Third, Dicer facilitates the formation of a large multiprotein complex called RISC (RNA-Induced Silencing Complex).
RISC can seek out and capture messenger RNA (mRNA) molecules with a base sequence complementary to that of its siRNA or miRNA.
This serves to either destroy the genetic message carried by the mRNA outright or else block the subsequent synthesis of a protein.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:3000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, pH 7.4. Reconstitute with 50ul ddH2O.
Specificity：Recognizes isoforms 1 and 2 of human DICER.
