HCV is a positive, single-stranded RNA virus in the Flaviviridae family.
The genome is ~10,000 nucleotides and encodes a single polyprotein of 3,000 amino acids.
The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non-structural protein necessary for viral replication.
Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha.
Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation.
Other applications not tested.
Recommended Dilution:ELISA: 1:1000Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes HCV NS5B, genotype 1B.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Immunogen：Recombinant protein corresponding to HCV NS5b.
