MaxLight™750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Binding of EPO to EPOR, results in the dimerization of the EPOR and the triggering of the JAK2/STAT5 signaling cascade, and in some cell types activation of STAT1 and STAT3.
Several isoforms of EPOR exist: EPOR-F, EPOR-S and EPOR-T, and a functional EPOR is found on endothelial cells and cardiomyocytes within the cardiovascular system, suggesting a role in cardiac function.
Applications:Suitable for use in FLISA, Western Blot, Immunoprecipitation.
Other applications not tested.
Recommended Dilution:FLISA: 50ng/mlWestern Blot: 10ug/ml Immunoprecipitation: 10ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™750 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™750.
Specificity：Recognizes Erythropoietin Receptor. Species Crossreactivity: Mouse
Isotype：IgG
Calc Applications Abbrev：FLISA IP WB
Calc Crossreactivity：Hu Mo
Immunogen：Partial cDNA of the extracellular domain of human EPOR expressed as a GST fusion protein in E. coli.
