MaxLight™750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Recommended Secondary Reagents: Sheep anti Rabbit IgG (Aff.
Pur.): HRP
Applications:Western Blotting: 1-2ug/mlOptimal working dilutions to be determined by researcher.
Western Blotting: Detects a band of approximately 35kD in HepG2 and 3T3 cell lysates.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™750 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified IgG was prepared from whole serum by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™750.
Specificity：Recognizes endonuclease G (endoG), a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis endoG i reported to act independently of caspases and may represent a caspase independent apoptotic pathway initiated from the mitochondria. Species Crossreactivity: Human. Expected crossreactivity from sequence similarity mouse, rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide GGPRGPGELAKYGLP corresponding to amino acids 55 - 70 of endonuclease G.
