MaxLight™750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Type IV collagen is a major component of the basement membrane and it associates with laminin, entactin and heparin sulfate proteoglycans to form sheet-like structures that separates epithelium from connective tissue.
It is a long triple helix containing several interruptions, which is characterized by a specific carboxylterminal, non-collagenous NC1 domain.
It is composed of six genetically different alpha (IV) chains, alpha1 (IV) to alpha 6(IV) and their genes are paired on three different chromosomes in a head-to-head arrangement.
The alpha 1 gene and the alpha 2 gene are localized on chromosome 13q34, the alpha 3 gene and the alpha 4 gene are on chromosome 2q36, and the alpha 5 gene and the alpha 6gene are on chromosome Xq22.
Type IV collagen can be used as a thin coating on tissue culture surfaces to promote cell attachment and proliferation and to study its effects on cell behavior.
Applications:Suitable for use in Immunofluorescence, Immunohistochemistry and FLISA.
Other applications have not been tested.
Recommended Dilution:Immunofluorescence: 1:40-1:160Immunohistochemistry (paraffin): 1:250-1:1000 (Reccommended Immunohistochemistry fixatives: AFA, buffered formalin (5-10%) or Bouin’s)FLISA: 1:100-1:400Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™750 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromotography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™750.
Specificity：Recognizes mouse collagen Type IV: 100%; mouse Types I, II, III: <0.1%; Human Types IV, V: <0.1%; Mouse Fibronectin: <0.1%; Mouse Laminin: <0.1%. Determined by RIA. Species Crossreactivity: rat and orangutan, reacts weakly with human.
Isotype：IgG
Calc Applications Abbrev：FLISA IF IHC
Calc Crossreactivity：Mo Rt
Immunogen：Native Collagen Type IV from mouse EHS tumor.
