84-3416-80 RIP3 (Receptor-interacting Serine/Threonine-protein Kinase 3, RIP-like Protein Kinase 3, RIPK3, Receptor-interacting Protein 3, RIP-3, mRIP3) (MaxLight 650) 100ul R2031-74G-ML650
特徴
MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection.
Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.
RIP3 is related to RIP and RIP2 but does not posses a death domain or CARD motif at its Cterminus.
RIP3 activates NFKappa B and potently induces apoptosis.
RIP3 is expressed in a range of tissues including the spleen, liver, testis, heart, brain and lung.
Applications:Suitable for use in Immunohistochemistry and Western Blot.
Other applications have not been tested.
Recommended Dilutions: Immunohistochemistry (paraffin): 5ug/ml.
Requires antigen retrieval using heat treatment prior to staining of paraffin sections
Western Blot: 0.5-1ug/ml detects a band of ~56kD in Mouse 3T3 whole cell lysateOptimal dilutions to be determined by the researcher.
Storage and Stability:Aliquot to avoid repeated freezing and thawing and freeze at -70°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Aliquots are stable for 6 months.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Source Antibody:mouse
Grade:Affinity Purified
Purity:Purified by affinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Specificity:Recognizes mouse RIP3. Species crossreactivity: rat.
Isotype:IgG
Calc Applications Abbrev:IHC WB
Calc Crossreactivity:Mo Rt
Immunogen:Synthetic peptide sequence corresponding to aa473-486 of mouse RIP3.
Shelf Life:1year
EU Commodity Code:30021010
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