84-3411-01 Caspase 12 (NT) (MaxLight 550) 100ul C2087-73-ML550
特徴
MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection.
Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.
Caspase 12 is a member of the interleukin-1 Beta converting enzyme (ICE) subfamily of caspases.
Caspase 12 is ubiquitously expressed in mouse tissue and is localized to the endoplasmic reticulum.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1ug/mlImmunohistochemistry (Paraffin): 10ug/ml; Requires antigen retrieval using heat treatment prior to staining.
Sodium citrate buffer, pH 6.0, is recommended
Optimal dilutions to be determined by the researcher.
Positive Control:Heart tissue (IHC)
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™550 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Source Antibody:mouse
Grade:Affinity Purified
Purity:Purified by affinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.
Specificity:Recognizes an epitope within the N-terminal region of mouse caspase 12. Species Crossreactivity: Human and rat.
Isotype:IgG
Calc Applications Abbrev:IHC WB
Calc Crossreactivity:Hu Mo Rt
Immunogen:Synthetic peptide corresponding to amino acids 2-17 of murine caspase 12.
Shelf Life:1year
Swiss Prot Number:O08736
EU Commodity Code:30021010
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