MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Interleukin-10 is produced by a population of T cells.
It has a wide range of biological properties, including inhibition of macrophage and T helper cell mediated cytokine synthesis suppression of the delayed hypersensivity response.
Reports suggest that IL-10 may initially be involved in the polarization the Th2 responses.
Applications:Suitable for use in FLISA, Western Blot and Neutralization.
Other applications not tested.
Recommended Dilution:FLISA: 0.5ug/mlWestern Blot: 0.1-0.2ug/mlNeutralization: 0.012-0.014ug/ml to yield one half maximal inhibition (ND50).
Optimal dilutions to be determined by the researcher.
Recommended Secondary Reagents:Sheep anti Rabbit IgG: HRP
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™490 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Source Antibody:mouse
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.