MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Growth Factor I, a 70aa secreted mitogenic polypeptide and member of the insulin gene family, produced primarily by the liver.
IGFI exhibits both autocrine and paracrine properties and has a widespread effect on cells including those of the bone, cartilage, muscle, nerves, skin, liverand lungs, playing a key role in cell proliferation and growth, inhibition of apoptosis and linked with tumor cell growth.
IGFI signals through binding to the type 1 insulinlike growth factor receptor (IGF1R) and also to the insulin receptor (IR), resulting in the activation of the AKT signalling pathway.
Production of IGFI is stimulated by growth hormone (GH) and can be hindered by GH insensitivity, deficiency in GH receptors or a malfunction in the post GH receptor signalling pathway.
Applications:Suitable for use in FLISA, Functional Assays, and Western Blot.
Other applications not tested.
Recommended Dilution:FLISA: 0.5-2ug/mlWestern Blot: 0.1-0.2ug/mlFunctional Assays: 0.67-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™490 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
