MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
ATG16 is a member of the autophagy protein family and forms a complex with the conjugate.
This multimeric protein has been shown to be essential for autophagosome formation in both yeast and mammals and targets the complex to the autophagic isolation membrane during the formation of the autophagosome.
Because mammalian ATG16 has seven WD-repeats in its C-Terminal domain, it has been suggested that these may form a platform for further protein-protein interactions
Multiple isoforms of ATG16 exist.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.25-0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™490 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
Specificity：Recognizes human ATG16. Species Crossreactivity: Mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to 18aa from human ATG16.
