MaxLight™405 is a new Violet photostable dye conjugate comparable to Alexa Fluor® 405, PacificBlue™, Brilliant™ Violet 421 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Ulk2 (Serine/threonine-protein kinase Ulk2), otherwise known as Unc-51-like kinase 2, is a widely expressed mammalian Atg1 homologue and member of the protein kinase superfamily, which shares structural similarity with a serine/threonine kinase found in C.
elegans, known to be involved in axonal elongation.
Studies have shown that murine Ulk2 and closely-related Ulk1, localize to autophagic isolation membranes under starvation conditions, and that the binding of Atg13 (Autophagy-related protein 13) to Ulk1 and Ulk2, stabilizes and activates them, and facilitates the phosphorylation of FIP200, a multifunctional protein involved in cell migration, proliferation, apoptosis, and tumor suppression.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: detects a band of ~130kD in HEK-293T cell lysates transfected with mouse ULK2
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™405 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™405.
Immunogen：KLH conjugated synthetic peptide corresponding to aa370-382 from mouse Ulk2. The corresponding sequence differs by 2aa in rat and 3aa in human. Species sequence homology: human and rat
