Platelet factor-4 (PF-4), otherwise known as CXCL4, is a member of the intercrine alpha (chemokine CXC) family, released from the alpha granules of activated platelets, during platelet aggregation.
PF-4 binds with high affinity to heparin, counteracting its’ anticoagulant effect, thereby promoting blood coagulation, and implicating PF-4 in wound repair.
PF-4 acts as a chemotactic factor for neutrophils, monocytes and fibroblasts, and binds wih CXCR3B, a splice variant of the chemokine receptor CXCR3.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Functional Assays.
Other applications not tested.
Recommended Dilution:ELISA: 0.5-2.0ug/mlWestern Blot: 0.1-0.2ug/mlImmunohistochemistry: paraffin, requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Sodium citrate buffer pH 6.0 is recommended
Functional Assays: 1.75-3.0ug/ml, for functional studies do not add sodium azideOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Reconstitute with 100ul sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).
Specificity：Recognizes human Platelet Factor-4.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human PF-4.
