PAK2 is a ubiquitously expressed, 64kD, serine/threonine protease found at higher levels in skeletal muscle, ovary, thymus and spleen.
p21-activated kinases (PAKs) bind activated Cdc42 and Rac via a Cdc42/Rac interactive binding (CRIB) domain.
Group 1 PAK kinases (PAK1, 2 and 3) regulate the activity of an adjacent conserved autoinhibitory domain and are associated with cellular processes, including cytoskeleton rearrangement, apoptosis and MAP kinase signalling.
Group 2 PAKs (PAK4, 5, and 6) lack the autoinhibitory domain and are not activated by Cdc42/Rac binding.
PAK2 has a cytostatic effect on cells and is activated in response to cellular stresses such hyperosmolarity, serum starvation, contact inhibition and DNA damage from radiation and chemicals.
Unlike other PAKs, PAK2 can be activated by proteolytic cleavage to generate a constitutively active fragment, PAK2p34, which induces a cell death response.
This induction is thought to be regulated by Rho GTPase-activating protein 10 which inhibits the protein kinase activity of PAK2p34 and causes it to localise to the perinuclear region instead of the nucleus.
PAK2 may also play a role in X-linked mental retardation.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).
Specificity：Recognizes human PAK2. Species Crossreactivity: mouse, rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：14 amino acid peptide sequence near the carboxy terminus of human PAK2.
