Myeloid cell leukemia sequence 1 (MCL-1) is an anti-apoptotic member of the Bcl-2 family of proteins, first identified in differentiating ML-1 myeloid cells, and expressed in many different cell lines, tissues and a variety of tumors.
The MCL-1 protein, localized to intracellular membranes and particularly mitochondrial membranes, has since been isolated in two distinct isoforms, known as MCL-1 long (MCL-1L) and MCL-1 short (MCL-1S).
MCL-1S has been identified as a pro-apoptotic splice variant of MCL-1L, missing the transmembrane and BH1 and BH2 (Bcl-2 homology) domains.
Interaction between these two antagonistic variants can act to regulate cell survival.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Highly Purified
Purity：Purified by Ion Exchange chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).
Specificity：Recognizes an epitope within the central region (IN) of human Myeloid cell leukemia sequence 1.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to a 16 amino acid peptide located near the centre of human MCL-1.
