84-3402-66　Macrophage, Monocyte Chemotactic Protein-1 (CCL2, Chemokine (C-C motif) ligand 2, C-C motif chemokine 2, GDCF-2, HC11, HSMCR30, MCAF, MCP1, MCP-1, MGC9434, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, SCYA2, Small-inducible cytokine A2, SMC-CF) (PE) 100ul　M1203-03G-PE

特徴

• MCP-1 is also known as CCL-2.
• Rat MCP-1 is a chemokine of approximately 12kD, which plays an important role in the inflammatory response of blood monocytes and tissue macrophages.
• Applications:Suitable for use in ELISA, Western Blot and most Functional Assays.
• Other applications not tested.
• Recommended Dilutions:ELISA: 0.5ug/mlWestern Blot: 0.1-0.2ug/mlFunctional Assays: 4-6ug/ml to yield one half maximal inhibition (ND50) of rat MCP-1.
• Do not add sodium azide
• Optimal working dilutions to be determined by researcher.
• Recommended Secondary Reagents:I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRbI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRbI1904-40J: IgG, Fc (HRP) Pab Gt xRbI1904-40P: IgG, Fc, X-Adsorbed (HRP) Pab Gt xRb
• Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
• Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.
• Note: Applications are based on unconjugated antibody.