Human Interleukin-1 receptor antagonist (IL-1RA), a naturally occurring inflammatory inhibitor protein, produced by monocytes, macrophages, neutrophils, corneal epithelial cells and fibroblasts, which inhibits the activity of IL-1 alpha and IL-1 beta, by competitively blocking binding to cell surface receptors for IL-1.
Genetic variations in IL-1RA gene (IL1RN) are associated with susceptibility to microvascular complications of diabetes type 4 (MVCD4), and IL-1RA has therapeutic applications in rheumatoid arthritis, asthma, psoriasis, sepsis, chronic myelogenous leukemia and inflammatory bowel disease.
May be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with a biotinylated human IL-1RA antibody as the detection reagent and recombinant human IL-1RA as the standard.
May used in Western Blotting applications under either reducing or non-reducing conditions with recombinant human IL-1RA as the positive control.
Applications:Suitable for use in ELISA, Western Blot, Functional Assays.
Other applications not tested.
Recommended Dilution:ELISA: 0.5-2ug/mlWestern Blot: 0.1-0.2ug/mlFunctional Assays: 2.5-3.7ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography from whole serum.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).
Specificity：Recognizes human Interleukin-1Ra.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human Interleukin-1Ra.
