Human IL-17F (interleukin-17F), a disulphide-linked homodimer consisting of two 133aa chains, which belongs to the IL-17 gene family and shares the highest sequence homology with IL-17A.
IL-17F is expressed by activated CD4+ T-cells and monocytes, and acts as a stimulator of cytokine production, including granulocyte colony-stimulating factor (GM-CSF), IL-6 and IL-8.
IL-7F also stimulates the production of IL-2, MCP-1 (monocyte chemotactic protein-1) and TGFB (Transforming growth factor beta) by endothelial cells, and inhibits endothelial cell angiogenesis
Has been used in neutralisation assays where 50% inhibition (ND50) of the biological activity of 50ng/ml of human IL-17F was achieved using 0.9-1.2ug/ml of antibody.
Applications:Suitable for use in ELISA, Western Blot, Functional Assays.
Other applications not tested.
Recommended Dilution:ELISA: 0.5-2ug/mlWestern Blot: 0.1-0.2ug/mlFunctional Assays: 0.9-1.2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).
Specificity：Recognizes human Interleukin-17F.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human Interleukin-17F.
