Interleukin-10 is produced by a population of T cells.
It has a wide range of biological properties, including inhibition of macrophage and T helper cell mediated cytokine synthesis suppression of the delayed hypersensivity response.
Reports suggest that IL-10 may initially be involved in the polarization the Th2 responses.
Applications:Suitable for use in ELISA, Western Blot and Neutralization.
Other applications not tested.
Recommended Dilution:ELISA: 0.5ug/mlWestern Blot: 0.1-0.2ug/mlNeutralization: 0.012-0.014ug/ml to yield one half maximal inhibition (ND50).
Optimal dilutions to be determined by the researcher.
Recommended Secondary Reagents:Sheep anti Rabbit IgG: HRP
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).
