84-3399-90　TIMP-1 (Tissue Inhibitor of Metalloproteinase-1, CLGI, TIMP, TIMP1) (HRP) 100ul　215178-HRP

特徴

• TIMP-1 (Tissue Inhibitor of Metalloproteinase -1), a secreted glycoprotein and member of the protease inhibitor I35 (TIMP) family, is expressed by a wide range of cells, including microvascular endothelial cells, smooth muscle cells, mesangial cells, and some fibroblasts.
• TIMP-1 acts as a natural extracellular inhibitor of MMPs (matrix metalloproteinases), a group of peptidases involved in degradation of the extracellular matrix, and is also involved in erythropoiesis, through the simulation of the proliferation/differentiation of erythroid progenitors.
• Studies have shown that a correlation between localized concentrations of TIMP-1 and TIMP-3, may determine cell fate, and that increased expression of TIMP-1, may act as an inhibitor/suppressor of invasive cell growth.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Functional Assays.
• Other applications not tested.
• Recommended Dilution:ELISA: 0.5-2.0ug/mlWestern Blot: 0.1-0.2ug/mlImmunohistochemistry (paraffin): requires enzyme mediated antigen retrieval prior to staining of parrafin sections.
• Proteinase K is recomended for this purpose
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.