Slug, a member of the Snail family of zinc finger transcription factors, whose expression is induced by FGF, BMP, and TGF-b, is a 29kD transcriptional repressor, which functions by binding to E-box motifs and inhibiting gene expression by recruiting histone deacetylase-1 (HDAC-1).
Slug controls the expression of a wide variety of genes including TP53, BRCA2, PUM and CDH1, and has important roles in apoptosis and epithelial-mesenchymal transition.
It is also thought to function in neural crest development.
Slug expression in breast, oesophageal, and colorectal carcinomas has been correlated with poor prognosis for survival.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human Slug at ~30kD in 293 cell lysate. Does not react with Snail. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：A 13aa peptide sequence near the center of human Slug.
