Presenilin enhancer protein 2 (PEN), a 12kD member of the PEN2 family and subunit of the gamma-secretase complex.
This complex is an endoprotease that cleaves integral membrane proteins.
These include Notch receptors and the beta-amyloid precursor protein which is cleaved to form amyloid beta peptide, a major component of the neuritic plaques found in Alzheimer’s disease.
The gamma secretase complex consists of nicastrin, the presenilin homodimers and APH1.
It is the addition of PEN2, the final stage of gamma secretase maturation that facilitates endoproteolysis of presenilin and confers proteolytic activity.
PEN2 expression has been observed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, skeletal muscle, heart and brain.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human PEN2. Species Crossreactivity: mouse, rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：13 amino acid peptide sequence near the amino terminus of human PEN2.
