84-3399-47　PIK3C3 (PI3-kinase Type 3, VPS34, Vacuolar Protein Sorting 34, Vps34) (HRP) 100ul　215082-HRP

特徴

• PS34 (vacuolar protein sorting 34), otherwise known as PI3-kinase type 3 (PIK3C3), is a ubiquitously expressed class III PI3K (phosphoinositide 3-kinase), and member of the PI3/PI4-kinase family, which specifically utilizes phosphatidylinositol (PtdIn) as a substrate, and functions as the catalytic subunit of the PI3K complex.
• VPS34 plays a role in the transport of lysosomal enzyme precursors to lysosomes, and in endocytosis and vesicular trafficking, and has been strongly implicated as a regulator of autophagy, forming a multi-protein complex with the proautophagic tumor suppressors Beclin1/Atg6, Bif-1, and UVRAG, in the initiation of autophagosome formation.
• Distinct complexes of VPS34 have been shown to regulate endocytic processes vital for late-stage autophagosome-lysosome fusion.
• PS34 has been tested in immunofluorescence using HUVEC cells, in conjunction with Hoescht 33342 (blue) nuclear staining.
• Applications:Suitable for use in Immunofluorescence and Western Blot.
• Other applications not tested.
• Recommended Dilution:Immunofluorescence: 1:100 Western Blot: 2-4ug/ml, detects a band of ~105kD in HEK-293T cell lysates
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.