Neurite Outgrowth Inhibitor (NogoA), also known as Reticulon-4 (RTN4), a member of a family of integral membrane proteins termed reticulons.
Research indicates that reticulons are involved in various neurodegenerative diseases.
Reticulon proteins have been demonstrated to regulate many cellular processes and interact with multiple proteins and receptors such as BACE.
NogoA was initially identified as a myelin-associated neurite outgrowth inhibitor.
It is highly expressed in oligodendrocytes in the white matter of the CNS.
Experimentation by blocking its activity with antibodies or other factors results in improved axon regrowth and functional recovery in experimental CNS lesion models.
NogoA has also been suggested to play a role in neurodegenerative diseases such as Amyotrophic lateral sclerosis,and multiple sclerosis.
Despite its predicted molecular weight, NogoA typically migrates at 180kD in an SDS-PAGE.
Five isoforms of Nogo have currently been identified.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions: Western Blot: 0.5-1ug/ml detects a band of ~180kD in mouse Brain tissue lysate
Immunohistochemistry (Paraffin): 2.5ug/ml.
Requires antigen retrieval using heat treatment prior to staining of paraffin embedded sections.
Sodium citrate buffer, pH 6.0 is recommended
Optimal dilutions to be determined by the researcher.
Immunohistochemistry Positive Control:Mouse brain
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affininty chromatography
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human NogoA. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to a 23aa sequence from near the carboxy terminus of human NogoA.
