Specifically Recognizes an epitope within the C-terminal (CT) region of endothelin B receptor (ET-B), a widely distributed transmembrane protein and member of the G-protein coupled receptor 1 family, which acts as a non-specific receptor for endothelin 1, 2, and 3, and mediates its action by G protein association and activation of a phosphatidylinositol-calcium second messenger system.
Recognizes epitopes on the cytoplasmic side of the receptor and therefore the cell membrane must be permeable to the antibody.
Does not block the binding of endothelins
Applications:Suitable for use in Immunofluorescence, Immunoprecipitation, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 10-20ug/mlImmunoprecipitation: 20-40ug/mlImmunohistochemistry(Paraffin): 10-20ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：sheep
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes rat Endothelin B Receptor. Species Crossreactivity: Mouse, human
Isotype：IgG
Calc Applications Abbrev：IF IHC IP
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to KANDHGYDNFRSSNN aa424-437 of rat Endothelin B Receptor, conjugated to KLH.
