The DISC1 protein is widely expressed in normal tissues.
It is involved in neurite outgrowth and cortical development through its interaction with a variety of proteins including lissencephaly-1 (LIS1), NudeE-like (NUDEL) and phosphodiesterase 4B.
In certain cases of schizophrenia the DISC1 gene has been found to be disrupted by a t(1;11) (q42.1;q14.3) translocation, and studies suggest that DISC1 could be a candidate susceptibility gene for this condition.
Applications:Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1-2ug/ml detects bands of approximately 95-100kD in SK-N-SH cell lysate.
Immunocytochemistry: 5ug/mlImmunohistochemistry: Frozen sectionsOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes disrupted in schizophrenia 1 (DISC1), 93kD protein located in the nucleus, cytoplasm and mitochondria. Species Crossreactivity: human and mouse
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：An 18 amino acid peptide located near the center of human DISC1.
