CXCL1, also known as Gro and KC., is an 8kD cytokine which is expressed predominantly by monocytes after activation, but it also expressed by fibroblasts, endothelial cells, synovia cell and several tumor cell lines.
CXCL1 promotes neutrophil chemotaxis and degranulation.
Applications:Suitable for use in ELISA, Western Blot and Functional Assays.
Other applications not tested.
Recommended Dilutions:ELISA: 0.5-2ug/mlWestern Blot: 0.1-0.2ug/mlFunctional Assays: 5-10ug/mlOptimal working dilutions to be determined by the researcher.
Recommended Secondary Reagents:I1904-40J: IgG, Fc (HRP) Pab Gt xRbSheep anti Rabbit IgG (HRP)
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O .
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Source Antibody:rat
Grade:Affinity Purified
Purity:Purified by affinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).