84-3397-50　［取扱停止］CD44v3 - v10 (CD44 Antigen, CD44 Molecule, Heparan Sulfate Proteoglycan, MUTCH 1, Phagocytic Glycoprotein I) (HRP) 100ul　C2398-91N-HRP

特徴

• CD44 is a ubiquitously expressed type 1 transmembrane glycoprotein and proteoglycan, otherwise known as phagocytic glycoprotein 1 (pgp 1), which acts as a receptor for the major extracellular matrix component hyaluronate, and also binds other ligands including collagen and matrix metalloproteinases (MMPs).
• CD44 is known to play a role in cell-cell interactions, cell adhesion, the activation and homing of lymphocytes, cell migration, hematopoiesis and metastasis, and exists as a number of isoforms generated by the alternative RNA splicing of at least 10 variant exons (CD44v).
• Applications:Suitable for use in Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Immunohistochemistry (Frozen): 50ug/mlImmunohistochemistry (Paraffin): 20ug/ml.
• Requires antigen retrieval using heat treatment prior to staining of paraffin sections.
• Use sodium citrate buffer pH 6.0
• Optimal dilutions to be determined by the researcher.
• Positive Control:Breast carcinoma and colon adenocarcinoma
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.