CD274 is expressed constitutively on macrophages and dendritic cells, and is induced on activated T-cells, B-cells, endothelial cells and epithelial cells in response to Interferons alpha, beta and gamma.
CD274 is reported to possess dual functions; inhibition of activated effector T cells and co-stimulation of naïve T cells.
CD274 inhibits proliferation of activated T cells via ligation to the co-inhibitory molecule CD279 (programmed death-1; PD-1) leading to the secretion of the regulatory cytokine interleukin-10.
CD274 has also been shown to co-stimulate early T cell priming and differentiation.
Deregulated CD274 function has been reported in chronic viral and intracellular bacterial infection, as well as in many autoimmune diseases and cancers.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-1ug/ml.
Detects a band of ~32kD in Raji cell lysate
Immunohistochemistry (Paraffin): 2.5ug/ml.
Requires antigen retrieval using heat treatment prior to staining paraffin sections.
Sodium citrate pH 6.0 is recommended
Optimal dilutions to be determined by the researcher.
