CCR8 (C-C chemokine receptor type 8), a transmembrane glycoprotein and member of the G-protein coupled receptor 1 family, which acts a high-affinity receptor for the chemokine I-309 (CCL1), implicated in the regulation of monocyte chemotaxis and thymocyte apoptosis.
CCR8 is constitutively expressed by monocytes, preferentially expressed on Th2 (type 2 helper T) cells, and acts as a co-receptor with CD4 for HIV1 infection.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100,000Western Blot: 1:1000Immunohistochemistry (paraffin): 1:400Optimal dilutions to be determined by the researcher.
Positive Control:Spleen
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes mouse CCR8.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide PNVTMTDYYPDFFTAPCDAEFLLRGSM corresponding to aa7-33 of mouse CCR8.
