84-3397-32　Caspase 4 (Caspase-4, CASP 4, CASP4, CASP-4, Apoptotic Cysteine Protease Mih1/TX, ICH 2, Ich-2, ICH2 Protease, Mih1/TX, TX, TX Protease) (HRP) 100ul　C2087-23V-HRP

特徴

• Caspase-4, a member of the Caspase-1 subfamily of cysteine proteases, exists as an inactive pro-enzyme which undergoes proteolytic cleavage of its p30 precursor form, into smaller p20 and p10 subunits.
• The cellular localisation of Caspase-4 on the Endoplasmic Reticulum (ER) membrane, has resulted in this protein becoming a focus of studies in which dysfunction or stress to the ER membrane is implicated, such as Alzheimer’s disease and Ischemia (1, 3) and confirms the involvement of Caspase-4 as an instigator of cellular apoptosis.
• Applications:Suitable for use in Immunohistochemistry and Western Blot.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry (paraffin): Does not require protein digestion pre-treatment or antigen retrieval using heat treatment prior to staining paraffin sections
• Western Blot: Detects a band at ~34kD in Ramos cell lysates
• Optimal dilutions to be determined by the researcher.
• Positive Control:Spleen tissue
• Recommended Secondary Antibody:I1903-30U: IgG, H&L, X-adsorbed (HRP)
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.