Bim is a pro-apoptotic protein within the Bcl-2 family, which functions within the intrinsic apotosis pathway.
Bim is known to interact with pro-survival proteins including Bcl-2, Bcl-XL and Bcl-w.
Bim is expressed in a variety of cell types, where it is maintained in an inactive conformation by phosphorylation or through binding to the microtubule-associated dynein motor complex.
Upon certain apoptotic stimuli, Bim is released from microtubules and mediates caspase-dependent apoptosis.
Applications:Suitable for use in Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: detects a band of ~22kD in K562 and A549 whole cell lysates.
Immunohistochemistry: Paraffin sections.
Requires antigen retrival using heat treatment prior to staining of paraffin sections.
Sodium citrate pH 6 is recommended for antigen retrival
Optimal dilution determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with Horseradish peroxidase (HRP).
Specificity：Recognizes human BIM.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa22-40 of human BIM. Species Sequence Homology: mouse and rat.
