Actins are highly conserved proteins that are involved in cell motility, structure and integrity and are ubiquitously expressed in all eukaryotic cells.
Six different isoforms of Actin have been identified, of these Actin beta is one of the three isoforms found in vertebrates.
Defects in Actin beta are a cause of juvenile dystonia, a condition that results in movement disorders due to disordered muscle tonicity, abnormal posture, development malformations and sensory hearing loss.
Applications:Suitable for use in Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1-2ug/mlImmunohistochemistry (Frozen): 1ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:HeLa cell lysates
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography from whole serum.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human ACTIN BETA (N-TERMINAL) at ~41.7kD. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to an 16aa sequence from near the amino terminus of human Actin beta.
