84-3394-91 Hrk (Activator of Apoptosis Harakiri, BH3-interacting Domain Containing Protein 3, BID3, DP5, Harakiri BCL2 Interacting Protein (Contains Only BH3 Domain), Neuronal Death Protein DP5) (FITC) 100ul H7446-50B-FITC
84-3394-91 Hrk (Activator of Apoptosis Harakiri, BH3-interacting Domain Containing Protein 3, BID3, DP5, Harakiri BCL2 Interacting Protein (Contains Only BH3 Domain), Neuronal Death Protein DP5) (FITC) 100ul H7446-50B-FITC
HRK regulates apoptosis through its interaction with the anti-apoptotic proteins Bcl-2 and Bcl-XL, and in conjunction to the mitochondrial pore forming protein p32.
HRK is thought to have a specific role in initiating neuronal and hematopoietic cell apoptosis, in particular to growth factor deprivation.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: Detects a band at ~13kD in mouse pancreas tissue lysate
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by affinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).
Specificity:Recognizes human Harakiri (HRK), also known as DP5. HRK is a BH3 only protein of the Bcl-2 family, which promotes Bax dependent apoptosis. Species Crossreactivity: mouse and rat
Isotype:IgG
Calc Applications Abbrev:WB
Calc Crossreactivity:Hu Mo Rt
Immunogen:Synthetic peptide corresponding to 15aa near the center of human HRK (NP_003797).