84-3393-87　［取扱停止］Calcitonin Receptor (FITC) 100ul　C0115-19-FITC

特徴

• Calcitonin receptor is a membrane protein with seven transmembrane domains that are coupled to G protein messenger systems.
• The calcitonin receptor is expressed in kidney, central and peripheral nervous systems and in osteoclasts.
• It is also expressed in a number of tissues during the development of the embyro.
• It may be important in fetal morphogenesis.
• Applications:Suitable for use in Immunohistochemistry, ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry (Paraffin): 1:10-1:100.
• The use of 4% paraformaldehyde fixation, rather than formalin fixation, is recommended.
• A sensitive detection system is also recommended; e.g.
• a catalyzed signal amplification system.
• Paraffin sections do not require protein digestion pre-treatment.
• Does not require antigen retrieval using heat treatment prior to staining
• ELISA: 1:10,000Western Blot: Reducing conditionsOptimal working dilutions to be determined by researcher.
• Recommended Positive Control Tissue:IHC: Kidney, cultered osteoclasts
• Recommended Secondary Reagent:I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: FITC conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.