CGRP is present in C-cells of the thyroid and in central and peripheral nerves.
CGRP has several important physiologic roles: it is a potent vasodilator, and can affect the force and rate of heart beat; it can modulate acetylcholine receptor function at the neuromuscular junction; it has been demonstrated to block tolerance to morphine; and it can modulate antigen presentation in Langerhans cells in the skin.
Despite these important physiologic functions, therapeutic strategies using CGRP have been impeded due to the lack of a cloned CGRP receptor with which ligands could be developed.
Applications:Suitable for use in FLISA, Immunofluorescence and Immunohistochemistry.
Not recommended for resin sections.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: Paraffin and frozen sectionsOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：goat
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes rat Calcitonin gene-related peptide, also known as CGRP. Reacts with both the whole molecule (aa1-37) and the C-terminal fragment (23-37). Species Crossreactivity: mouse, guinea pig and emu.
Isotype：IgG
Calc Applications Abbrev：FLISA IF IHC
Calc Crossreactivity：Gp Mo Rt
Immunogen：Synthetic peptide corresponding to aa23-37 of rat Tyr-CGRP conjugated to gamma globulin.
