Stem cell factor (SCF), is a type I transmembrane protein and haematopoietic growth factor which acts in synergy with other growth factors, and signals through the receptor tyrosine kinase c-Kit (CD117).
The interaction between SCF and c-Kit regulates developmental and functional processes of haematopoietic progenitor cells, mast cells, myelanocytes and germ cells, and plays an important role in follicular development.
The interaction between SCF and c-Kit is associated with the progression and invasiveness of some tumors, including melanomas.
Applications:Suitable for use in ELISA, Western Blot and Functional Assays.
Other applications not tested.
Recommended Dilution:ELISA: 0.5-2.0ug/mlWestern Blot: 0.1-0.2ug/mlFunctional Assays: 0.08-0.12ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with 100ul sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Source Antibody:rat
Grade:Affinity Purified
Purity:Purified by affinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Specificity:Recognizes rat Stem Cell Factor.
Isotype:IgG
Calc Applications Abbrev:E WB
Calc Crossreactivity:Rt
Immunogen:Recombinant protein corresponding to rat SCF.