Slug, a member of the Snail family of zinc finger transcription factors, whose expression is induced by FGF, BMP, and TGF-b, is a 29kD transcriptional repressor, which functions by binding to E-box motifs and inhibiting gene expression by recruiting histone deacetylase-1 (HDAC-1).
Slug controls the expression of a wide variety of genes including TP53, BRCA2, PUM and CDH1, and has important roles in apoptosis and epithelial-mesenchymal transition.
It is also thought to function in neural crest development.
Slug expression in breast, oesophageal, and colorectal carcinomas has been correlated with poor prognosis for survival.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Specificity：Recognizes human Slug at ~30kD in 293 cell lysate. Does not react with Snail. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：A 13aa peptide sequence near the center of human Slug.
