84-3391-86　Interleukin 12 (IL-12, Cytotoxic Lymphocyte Maturation Factor 1, CTL Maturation Factor, TcMF, CLMF p35, Natural Killer Cell Stimulatory Factor 1, NFSK1, p35, T cell Stimulating Factor, TSF) (Biotin) 100ul　I8434-08C-Biotin

特徴

• Interleukin-12 is produced by activated B lymphocytes and macrophages.
• The properties of IL-12 include the stimulation of growth of activated T lymphocytes and the development of TH1 CD4 positive cells and CD8 positive cytotoxic T cells.
• Applications:Suitable for use in ELISA (direct), Western Blot and Neutralization.
• Other applications not tested.
• Recommended Dilution:ELISA: 0.5ug/ml
• Western Blot: 0.1-0.2ug/ml
• Neutralization: 0.012-0.20ug/ml to yield one half maximal inhibition (ND50) of mouse IL-12.
• Optimal working dilutions to be determined by researcher.
• Recommended Secondary Reagents:Donkey anti-sheep IgG, H&L (HRP): I1904-63Donkey anti-goat IgG, H&L (HRP): I1904-28C
• Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
• Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.
• Note: Applications are based on unconjugated antibody.