Interleukin10, an 18.5kD immunosuppressive cytokine originally known as cytokine synthesis inhibiting factor (CSIF), which specifically regulates immunemediated, inflammation and shareshigh sequence homology with murine IL10 and the viral protein BCRF1.
IL10, produced by macrophages, mast cells, T cells, activated B cells and keratinocytes, inhibits the secretion of avariety of proinflammatory cytokines including TNFalpha, IL1, IL2, IL3 and IL8 and is chemotactic for CD8+ T cells.
The suppressive ability of IL10 has become a focus area for the treatment of Crohn’s disease, but with conflicting results.
Applications:Suitable for use in ELISA, Functional Assays, and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.5ug/mlWestern Blot: 0.1-0.2ug/mlFunctional Assays: 4-6ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
