Insulinlike Growth Factor II, a 67aa mitogenic polypeptide and member of the Insulin gene family, synthesized by various foetal tissues during prenatal development, under the influence of placental lactogen.
IGFII is a growth factor which exhibits both paracrine and autocrine properties and signals by binding to the type 1 and type 2 insulinlike growth factor receptors (type 1 IGFR and type 2 IGFR), and also to the insulin receptor (IR).
The availability of IGFII is dependent upon its attachment to, and release from, various IGF binding proteins (IGFBPs), a relationship which has been investigated in breast cancer research (2).
Applications:Suitable for use in ELISA, Functional Assays, and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.5ug/mlWestern Blot: 0.1-0.2ug/mlFunctional Assays: 3.1-4.6ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
