GAPDH is a major glycolytic enzyme within the cytosol, which is also involved in a number of intracellular processes including membrane fusion, microtubule bundling, phosphotransferase, DNA replication and DNA repair.
Recent studies have suggested that GAPDH may be involved in the cellular phenotype of age-related neurodegenerative disorders such as Alzheimer’s and Huntington’s disease.
Applications:Suitable for use in Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (Paraffin): Requires antigen retrival using heat treatment prior to staining of paraffin sections.
Sodium citrate buffer pH 6 is recommended
Optimal dilutions to be determined by the researcher.
Histology Positive Control Tissue:Human liver tissue
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Specificity：Recognizes human GAPDH. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A peptide corresponding to a 16aa sequence from near the N-terminus of human GAPDH.
