FLICE-inhibitory proteins (FLIP) are widely expressed apoptosis regulator proteins.
FLIPs may function as a crucial link between cell survival and cell death pathways in mammalian cells.
At least 14 isoforms of FLIP have been defined arising from alternative splicing.
The long form FLIP-L contains a domain similar to those found in caspases.
FLIP was cloned independently in a number of laboratories and is also known as Casper, I-FLICE, FLAME-1, CASH and CLARP.
FLIP has two death effector domains (DEDs) and a caspase-like domain.
It interacts with FADD and caspase-8 and -10, potently inhibiting apoptosis by all known death receptors.
FLIP exists in a short form (FLIPS) and a long form (FLIPL).
Applications: Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: Frozen sectionsOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Grade：Highly Purified
Purity：Purified by Ion exchange chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Specificity：Recognizes the 55kD FLIPL protein, a FLICE inhibitory protein. Does not recognize FLIPS (FLIP short form).
